Alpha-2,3-Sialidase

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Catalog Number

LDG0024RG

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• Overview

  Description

  Alpha-2,3-sialidase is an enzyme that specifically cleaves sialic acid residues linked to glycoproteins or glycolipids via an α-2,3 linkage. This glycosidase plays a crucial role in the modification and degradation of sialic acids, which are important for various biological processes, including cell signaling, immune response, and pathogen recognition. Alpha-2,3-sialidase activity is essential in studying viral infections, such as influenza, where the removal of sialic acid residues can affect viral binding and entry into host cells.

• Specifications

  • Expression System

    Escherichia coli

    Concentration

    50 U/μL

  • Storage Buffer

    20 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA, pH 7.5

    Purity

    >95% as determined by SDS-PAGE analysis.

  • Unit Definition

    One unit is defined as the enzyme required to cleave > 95% of the terminal α-Neu5Ac from 1 nanomole Neu5Ac-GalGalNAc of glycoprotein in 1 hour at 37°C in 40 μL reaction buffer (50 mM Tris-HCl, 100 mM NaCl, pH 7.5).

    Endotoxin Level

    <1 EU per 1 μg of the protein by the LAL method

  • Form

    Liquid

• Instruction

  • Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    Stability & Storage

    This product is stable after storage at:

    • -20°C for -80°C long-term storage under sterile conditions.

    Avoid repeated free-thaw cycles.

• Image

  The standard assay was performed by incubating 1 unit of alpha-2,3-sialidase and 1 nanomole of Fetuin under the above conditions.
  SDS-PAGE analysis of Fetuin digested with alpha-2,3-sialidase.

  SDS-PAGE analysis of recombinant alpha-2,3-sialidase.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.