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Catalog Number

LDG0045YA

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Overview

Description

The c-mesenchymal epithelial transition factor (c-Met；also known as hepatocyte growth factor receptor, HGFR), is a receptor tyrosine kinase (RTK) that mainly exists in epithelial cells. c-Met and its high-affinity ligand, hepatocyte growth factor (HGF), play important roles in mediating embryogenesis, tissue regeneration, wound healing and the formation of nerve and muscle. Aberrant HGF/c-Met axis activation is associated with the proliferation, survival, invasion and metastasis of various tumor cells, and thus c-Met may be a tumor biomarker and therapeutic target.

Product Note

Recommended dilution factor:
ELISA: 1:5000-20000
WB: 1:1000-10000
IFA: 1:200-1000
FACS: Assay dependent

Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.
Specifications
- Host
  
  Mouse
  
  Clonality
  
  Monoclonal
- Isotype
  
  IgG1
  
  Clone Name
  
  clone 4A9
- Immunogen
  
  c-Met
  
  Reactivity
  
  Human
- Application
  
  ELISA, WB, IFA, FACS
  
  Conjugation
  
  Unconjugated
- Concentration
  
  1 mg/mL
  
  Storage Buffer
  
  Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.
- Specificity
  
  c-Met
  
  Form
  
  Liquid
Instruction
- Shipping
  
  The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
  Stability & Storage
  
  This product is stable after storage at:
  - 2-8°C for 2 weeks under sterile conditions from date of receipt.
  - -20°C or -80°C for 12 months under sterile conditions from date of receipt.
  Avoid repeated freeze/thaw cycles.
  Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.
Image

Immunofluorescence analysis of Anti-c-Met Antibody [Clone 4A9]
MCF7 cells were fixed in 100% methanol, permeabilized with PBS containing
0.1% Triton X-100. Cells were stained with mouse anti-c-Met monoclonal
antibody (1:200) followed by secondary antibodies (goat anti-Mouse IgG-
iFluor 488, 1:200, green) and cell nuclei were stained with Hoechst 33342
(Blue).

FACS analysis of Anti-c-Met Antibody [Clone 4A9]
MCF-7 cells were stained with anti-c-Met monoclonal antibody at 2 μg/ml
(red) and without antibody control (black).

ELISA titration of Anti-c-Met Antibody [Clone 4A9]
Titration curve of anti-c-Met antibody in ELISA. Red: c-Met; Black: BSA
(negative control).

Western blotting analysis of Anti-c-Met Antibody [Clone 4A9]
HeLa cell lysates (50 μg) were stained with mouse anti-c-Met monoclonal
antibody at 1:5000 dilution.

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Datasheet & Documents

Datasheet

MSDS

Manual

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