Anti-CD326 Antibody [Clone 6G2]

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Catalog Number

LDG0072YA

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• Overview

  Description

  Mouse anti-human CD326 mAb recognizes CD326, also known as Epithelial Cell Adhesion Molecule (EpCAM). CD326 expression has been reported to be a possible marker of early malignancy, with expression being increased in tumor cells.

  Product Note

  Recommended dilution factor:
  ELISA: 1:5000-20000
  WB: 1:1000-5000
  IFA: 1:500-1000
  FACS: 1:500-1000
  
  Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

• Specifications

  • Host

    Mouse

    Clonality

    Monoclonal

  • Isotype

    IgG2a

    Clone Name

    clone 6G2

  • Immunogen

    MDA-MB-231 adenocarcinoma cell line

    Reactivity

    Human

  • Application

    ELISA, WB, IFA, FACS

    Conjugation

    Unconjugated

  • Concentration

    1 mg/mL

    Storage Buffer

    Phosphate Buffered Saline, pH 7.4.

  • Specificity

    CD326

    Form

    Liquid

• Instruction

  • Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at 2-8°C or lower for long term storage.

    Stability & Storage

    This product is stable after storage at:

    • 2-8°C for 6 months under sterile conditions from date of receipt.

    Briefly centrifuge vials before opening.

• Image

  Immunofluorescence analysis of anti-CD326 mAb
  SW480 cells were fixed in 4% PFA. Experimental cells were stained with
  mouse anti-human CD326 monoclonal antibody (1:500) followed by
  secondary antibodies (goat anti-Mouse IgG-iFluor 488, 1:400, green). All cell
  nuclei were stained with Hoechst 33342 (blue).

  Flow Cytometry analysis of anti-CD326 mAb
  MDA-MB-231 and Jurkat cells were stained with mouse anti-human CD326
  monoclonal antibody at 1 μg/ml (red) and without antibody control (black).

  Western blotting analysis of anti-CD326 mAb (1:1000)
  SW480 cell lysate (non-reduced) (30 μg)

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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