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Leadgene Leadgene
Anti-DENV Capsid Antibody [Clone 25-4]
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Catalog Number

LDG0014YA

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  • Overview

    Description

    Dengue virus belongs to the family Flaviviridae, genus Flavivirus and consists of four distinct serotypes (DENV1 to 4). Dengue virus is transmitted by mosquitos majorly of species Aedes aegypti and Aedes Albopictus that widespread around tropics and subtropics. Capsid protein is a small protein with very particular structural features, playing an integral role in replication and pathogenesis. Capsid protein interacts with host components, and thus is the potential drug target against DENV infection.

    Product Note

    Recommended dilution factor:
    ELISA: 1:5000-20000
    WB: 1:1000-5000
    IFA: 1:500-1000
    FACS: Assay dependent

    Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

  • Specifications
    • Host

      Mouse

      Clonality

      Monoclonal

    • Isotype

      IgG2b

      Clone Name

      clone 25-4

    • Immunogen

      Dengue virus Capsid

      Reactivity

      Dengue virus

    • Application

      ELISA, WB, IFA, FACS

      Conjugation

      Unconjugated

    • Concentration

      1 mg/mL

      Storage Buffer

      Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

    • Specificity

      capsid

      Form

      Liquid

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8°C for 2 weeks under sterile conditions from date of receipt.
      • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

      Avoid repeated freeze/thaw cycles.
      Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

  • Image
    ELISA titration of DENV Capsid antibody

    ELISA titration of DENV Capsid antibody

    Western blotting analysis of DENV Capsid antibody (1:1000) Lane 1: ZIKV-infected C636 cell lysate Lane 2: DENV 1-infected C636 cell lysate Lane 3: DENV 2-infected C636 cell lysate Lane 4: DENV 3-infected C636 cell lysate Lane 5: DENV 4-infected C636 cell lysate Lane 6: C636 cell lysate

    Western blotting analysis of DENV Capsid antibody (1:1000)
    Lane 1: ZIKV-infected C636 cell lysate
    Lane 2: DENV 1-infected C636 cell lysate
    Lane 3: DENV 2-infected C636 cell lysate
    Lane 4: DENV 3-infected C636 cell lysate
    Lane 5: DENV 4-infected C636 cell lysate
    Lane 6: C636 cell lysate

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  • Datasheet & Documents
    MSDS
    Manual
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