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Leadgene Leadgene
Anti-GAPDH Antibody [Clone 38-1]
Catalog Number

LDG0009YC

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  • Overview

    Description

    Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a catalytic enzyme in glycolysis. GAPDH exists as a tetramer of 37 kDa and is expressed at high level in most tissues. It is useful as internal control in western blotting.

    Product Note

    Recommended dilution factor:
    ELISA: 1:5000-20000
    WB: 1:1000-10000
    IFA: 1:200-1000

    Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

  • Specifications
    • Host

      Mouse

      Clonality

      Monoclonal

    • Isotype

      IgG2b

      Clone Name

      clone 38-1

    • Immunogen

      Recombinant GAPDH protein

      Reactivity

      Mouse, Rat, Human, Hamster, Monkey

    • Application

      ELISA, WB, IFA

      Conjugation

      Unconjugated

    • Concentration

      1 mg/mL

      Storage Buffer

      Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

    • Form

      Liquid

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8°C for 2 weeks under sterile conditions from date of receipt.
      • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

      Avoid repeated freeze/thaw cycles.
      Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

  • Image
    Western blotting analysis of anti-GAPDH mAb (1:2000) Lane 1: Rat liver lysate Lane 2: Mouse lung lysate Lane 3: BHK cell lysate Lane 4: Vero cell lysate Lane 5: 293T cell lysate Lane 6: 3T3 cell lysate 30 μg lysate per lane

    Western blotting analysis of anti-GAPDH mAb (1:2000)
    Lane 1: Rat liver lysate
    Lane 2: Mouse lung lysate
    Lane 3: BHK cell lysate
    Lane 4: Vero cell lysate
    Lane 5: 293T cell lysate
    Lane 6: 3T3 cell lysate
    30 μg lysate per lane

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  • Datasheet & Documents
    MSDS
    Manual
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Disclaimer:For Research Use or Further Manufacturing Only.

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