Anti-mCherry Antibody [Clone 45-5]

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Catalog Number

LDG0007YB

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• Overview

  Description

  The mCherry protein is a basic red fluorescent protein derived from Discosoma sp. Leadgene mouse anti-mCherry mAb can recognize native and denature mCherry protein but not GFP.

  Product Note

  Recommended dilution factor:
  ELISA: 1:5000-20000
  WB: 1:1000-5000
  IP: 1:500-2000
  IFA: 1:500-1000
  FACS: 1:500-1000
  
  Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

• Specifications

  • Host

    Mouse

    Clonality

    Monoclonal

  • Isotype

    IgG2a

    Clone Name

    clone 45-5

  • Immunogen

    Recombinant mCherry protein

    Application

    ELISA, WB, IP, IFA, FACS

  • Conjugation

    Unconjugated

    Concentration

    1 mg/mL

  • Storage Buffer

    Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

    Specificity

    Recognizes mCherry but not GFP.

  • Form

    Liquid

• Instruction

  • Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    Stability & Storage

    This product is stable after storage at:

    • 2-8°C for 2 weeks under sterile conditions from date of receipt.
    • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

    Avoid repeated freeze/thaw cycles.
    Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

• Image

  Immunofluorescence analysis of anti-mCherry mAb (1:500)

  Flow cytometry analysis of anti-mCherry mAb (1:1000)

  Western blotting analysis of anti-mCherry mAb (1:2000)
  Lane 1: 293T cell lysate
  Lane 2: 293T-Flag mCherry cell lysate
  Lane 3: GFP fusion protein
  Lysate/protein at 6 μg per lane

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.