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Catalog Number

LDG0054YA

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Overview

Description

Pepsinogens are members of the peptidases that are secreted by gastric chief cells and are inactive polypeptide proenzymes known as zymogens. They are converted by gastric acid to active pepsins, which are involved in the digestion of proteins. Pepsinogen A5 (PGA5), an isozymogen of pepsinogen A, is the predominant endopeptidase in the gastric acid and is associated with atrophic gastritis and gastritis.

Product Note

Recommended dilution factor:
ELISA: 1:5000-20000
WB: 1:1000-5000
IFA: 1:100-1000
FACS: Assay dependent

Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.
Specifications
- Host
  
  Mouse
  
  Clonality
  
  Monoclonal
- Isotype
  
  IgG1
  
  Clone Name
  
  clone 85-5
- Immunogen
  
  The immunogen information used to generate this antibody is proprietary.
  
  Reactivity
  
  Human
- Application
  
  ELISA, WB, IFA, FACS
  
  Conjugation
  
  Unconjugated
- Concentration
  
  1 mg/mL
  
  Storage Buffer
  
  Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.
- Specificity
  
  PGA5
  
  Form
  
  Liquid
Instruction
- Shipping
  
  The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
  Stability & Storage
  
  This product is stable after storage at:
  - 2-8°C for 2 weeks under sterile conditions from date of receipt.
  - -20°C or -80°C for 12 months under sterile conditions from date of receipt.
  Avoid repeated freeze/thaw cycles.
  Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.
Image

Immunofluorescence analysis of Anti-PGA5 Antibody [Clone 85-5]
HeLa cells were fixed in 4% PFA, permeabilized with PBS containing 0.1%
Triton X-100. Cells were stained with mouse anti-PGA5 monoclonal antibody
(1:100) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488,
1:200, green) and cell nuclei were stained with Hoechst 33342 (Blue).

FACS analysis of Anti-PGA5 Antibody [Clone 85-5]
K562 cells were stained with mouse anti-PGA5 monoclonal antibody at 2
μg/ml (red) and without antibody control (black).

ELISA analysis of Anti-PGA5 Antibody [Clone 85-5]
The specificity of anti-PGA5 antibody recognize recombinant proteins in
ELISA. Red: Porcine pepsin; Orange: PGA5; Green: Pepsinogen I; Blue:
Pepsinogen II; Black: BSA (negative control).

Western blotting analysis of Anti-PGA5 Antibody [Clone 85-5]
Recombinant proteins and mouse stomach lysates were stained with mouse
anti-PGA5 monoclonal antibody at 1:5000 dilution. Lane 1: Purified human
pepsinogen I (25 ng). Lane 2: Purified human pepsinogen II (25 ng). Lane 3:
Mouse stomach lysate (30 μg). Lane 4: Human gastric juice (10 μL).

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Datasheet & Documents

Datasheet

MSDS

Manual

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