Description
Bst DNA Polymerase (Large fragment) is an enzyme of Bacillus stearothermophilus DNA polymerase which can catalyze 5´ → 3´ polymerase activity but lacks 5´ →3´ exonuclease activity. Bst DNA Polymerase offers strand displacement capabilities, making it ideal for isothermal amplification.
Product Note
- It is not recommended to perform reaction above 70 °C. Bst DNA Polymerase cannot be used for thermal cycle sequencing.
- 10X Bst DNA Polymerase Reaction Buffer: 200 mM Tris-HCl (pH 8.8), 10 mM (NH₄)₂SO₄, 10 mM KCl, 20 mM MgSO₄, and 1% Triton® X-10.
Components
1,600 U
Items
Bst DNA Polymerase (Large Fragment) (8 U/μL)
Quantity
1 vial (1,600 U)
Items
10× Bst DNA Polymerase Reaction Buffer
Quantity
1 vial (1 mL)
Items
100 mM MgSO₄
Quantity
1 vial (0.4 mL)
8,000 U
Items
Bst DNA Polymerase (Large Fragment) (8 U/μL)
Quantity
1 vial (8,000 U)
Items
10× Bst DNA Polymerase Reaction Buffer
Quantity
1 vial (1 mL)
Items
100 mM MgSO₄
Quantity
1 vial (0.4 mL)
