Language
EN
TW
Leadgene Leadgene
Cholesterol Oxidase (CO)
More Images
Catalog Number

LDG0025RG

Datasheet
More Documents
Loading package & price...
COA Search
  • Overview

    Description

    Cholesterol oxidase (CO) is a bacterial enzyme that catalyzes the oxidation of cholesterol to cholest-4-en-3-one, producing hydrogen peroxide as a byproduct. This enzyme is widely used in clinical diagnostics for measuring cholesterol levels in blood samples, as it plays a crucial role in cholesterol metabolism. Additionally, cholesterol oxidase is used in research to study cholesterol biosynthesis, and in the biotechnology industry for the production of steroids and as a biocatalyst in biosensors. It is primarily sourced from species such as Streptomyces and Brevibacterium.

  • Specifications

    • Expression System

      Escherichia coli

      Activity

      Please refer to the manual for details.

    • Unit Definition

      One unit causes the formation of one micromole of hydrogen peroxide (half a micromole of quinoneimine dye) per minute under the conditions detailed below.
      (87 mM Potassim phosphate buffer, 0.89 mM Cholesterol, 1.4 mM 4-Aminoantipyrine, 21 mM Phenol, 0.34 % Triton X-100, 64 mM Sodium cholate, 33 μg/ mL BSA, 5 U/ mL Peroxidase)

      Reaction Condition

      87 mM Potassim phosphate buffer, 0.89 mM Cholesterol, 1.4 mM 4-Aminoantipyrine, 21 mM Phenol, 0.34 % Triton X-100, 64 mM Sodium cholate, 33 μg/ mL BSA, 5 U/ mL Peroxidase

    • Form

      Lyophilized

  • Instruction

    • Reconstitution

      It is recommended to weight 10 mg of lyophilized powder, reconstitute in 1 mL double-distilled water directly, and incubate the solution for at least 10 mins to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable at -20°C for long-term storage under sterile conditions.
      Avoid repeated free-thaw cycles.

  • Image
    Temperature activity of Cholesterol oxidase. The enzyme reactions in 0.1 M Potassium phosphate buffer, pH 7.0, were carried out under different temperature

    Temperature activity of Cholesterol oxidase.
    The enzyme reactions in 0.1 M Potassium phosphate buffer, pH 7.0, were carried out under different temperature

    pH activity of Cholesterol oxidase. The buffer conditions with various pH values were used in the reaction at 37°C. pH 4.0-6.0, 0.1 M Sodium citrate buffer; pH 7.0-8.0, 0.1 M Potassium phosphate buffer; pH 10.0-11.0, 0.1 M Carbonate-bicarbonate buffer.

    pH activity of Cholesterol oxidase.
    The buffer conditions with various pH values were used in the reaction at 37°C. pH 4.0-6.0, 0.1 M Sodium citrate buffer; pH 7.0-8.0, 0.1 M Potassium phosphate buffer; pH 10.0-11.0, 0.1 M Carbonate-bicarbonate buffer.

    Thermal stability of Cholesterol oxidase. The enzyme powder was reconstituted by double-distilled water and treated with different temperature for 15 minutes. Final concentration: 4.3 U/ mL.

    Thermal stability of Cholesterol oxidase.
    The enzyme powder was reconstituted by double-distilled water and treated with different temperature for 15 minutes. Final concentration: 4.3 U/ mL.

    pH stability of Cholesterol oxidase. The enzyme powder was reconstituted by double-distilled water and treated with different pH buffer condition for 20 hours. pH 4.0-6.0, 0.1 M Sodium citrate buffer; pH 7.0-8.0, 0.1 M Potassium phosphate buffer; pH 8.5, 0.1 M Tris-HCl buffer; pH 10.0-11.0, 0.1 M Carbonate-bicarbonate buffer.

    pH stability of Cholesterol oxidase.
    The enzyme powder was reconstituted by double-distilled water and treated with different pH buffer condition for 20 hours. pH 4.0-6.0, 0.1 M Sodium citrate buffer; pH 7.0-8.0, 0.1 M Potassium phosphate buffer; pH 8.5, 0.1 M Tris-HCl buffer; pH 10.0-11.0, 0.1 M Carbonate-bicarbonate buffer.

    1
    /
    4
    Total   (4)
  • Review

    Help others learn more about this product. Use the link below to share your experience.

    Submit a Review
  • Publication

    There are currently no publications. Use the link below to let us know.

    Submit a Publication
  • Datasheet & Documents
    Datasheet
    MSDS
    Manual
    1
    /
    2

Disclaimer：For Research Use or Further Manufacturing Only.

This site uses cookies as described in our Privacy Policy in order to improve your user experience and deliver customized advertising to you. Click “I Agree” to consent and continue.