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D-3-Hydroxybutyrate Dehydrogenase (3-HBDH)
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Catalog Number

LDG0022RG

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  • Overview

    Description

    D-3-Hydroxybutyrate dehydrogenase (3-HBDH) is an enzyme crucial in the metabolism of ketone bodies, specifically involved in the conversion of D-3-hydroxybutyrate to acetoacetate during ketogenesis. 3-HBDH plays a vital role in energy production, particularly under conditions such as fasting or prolonged exercise when ketone bodies serve as alternative energy sources. This enzyme is essential for maintaining metabolic balance and is found in various tissues, including the liver and kidneys. Understanding 3-HBDH's function is significant for insights into metabolic disorders and energy metabolism regulation.

  • Specifications

    • Expression System

      Escherichia coli

      Detection Method

      Spectrophotometry

    • Concentration

      ≥300 U/ mg

      Activity

      Please refer to the manual for details.

    • Unit Definition

      One unit is defined as the formation of one micromole of NADH per minute at 37°C under the conditions described below.
      (0.1 M Tris-HCl pH 8.5, 25 mM 3-Hydroxybutyrate, 1.8 mM NAD+).

      Reaction Condition

      0.1 M Tris-HCl pH 8.5, 25 mM 3-Hydroxybutyrate, 1.8 mM NAD+

    • Form

      Lyophilized

  • Instruction

    • Reconstitution

      It is recommended to weight 10 mg of lyophilized powder, reconstitute in 250 μL double-distilled water directly (final activity is 40 U/ μL), and incubate the solution for at least 10 mins to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable at -20°C for long-term storage under sterile conditions.
      Avoid repeated free-thaw cycles.

  • Image
    Temperature activity of 3-HBDH. The enzyme reactions in 0.1 M Tris-HCl buffer, pH 8.5, were carried out under different temperature.

    Temperature activity of 3-HBDH.
    The enzyme reactions in 0.1 M Tris-HCl buffer, pH 8.5, were carried out under different temperature.

    pH activity of 3-HBDH. The buffer conditions with various pH values were used in the reaction at 37°C. pH 4.0-6.0, 0.1 M Sodium citrate buffer; pH 7.0-8.0, 0.1 M Potassium phosphate buffer; pH 8.5, 0.1 M Tris-HCl buffer; pH 10.0-11.0, 0.1 M Carbonate-bicarbonate buffer.

    pH activity of 3-HBDH.
    The buffer conditions with various pH values were used in the reaction at 37°C. pH 4.0-6.0, 0.1 M Sodium citrate buffer; pH 7.0-8.0, 0.1 M Potassium phosphate buffer; pH 8.5, 0.1 M Tris-HCl buffer; pH 10.0-11.0, 0.1 M Carbonate-bicarbonate buffer.

    Thermal stability of 3-HBDH. The enzyme powder was reconstituted by double-distilled water and treated with different temperature for 15 minutes. Final concentration: 15.9 U/ mL.

    Thermal stability of 3-HBDH.
    The enzyme powder was reconstituted by double-distilled water and treated with different temperature for 15 minutes. Final concentration: 15.9 U/ mL.

    pH stability of 3-HBDH. The enzyme powder was reconstituted by double-distilled water and treated with different pH buffer condition for 20 hours. pH 4.0-6.0, 0.1 M Sodium citrate buffer; pH 7.0-8.0, 0.1 M Potassium phosphate buffer; pH 8.5, 0.1 M Tris-HCl buffer; pH 10.0-11.0, 0.1 M Carbonate-bicarbonate buffer.

    pH stability of 3-HBDH.
    The enzyme powder was reconstituted by double-distilled water and treated with different pH buffer condition for 20 hours. pH 4.0-6.0, 0.1 M Sodium citrate buffer; pH 7.0-8.0, 0.1 M Potassium phosphate buffer; pH 8.5, 0.1 M Tris-HCl buffer; pH 10.0-11.0, 0.1 M Carbonate-bicarbonate buffer.

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  • Datasheet & Documents
    Datasheet
    MSDS
    Manual
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