dCas9-VP64

Catalog Number

LDG003POE

Datasheet

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• Specifications

  • Species of Origin

    Streptococcus pyogenes

    Expression System

    Escherichia coli

  • Affinity Tag

    His Tag (C-term)

    Storage Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

  • Purity

    >90% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 169.29 kDa.
    The protein migrates as 130-180 kDa under reducing condition (SDS-PAGE analysis).

  • Endotoxin Level

    <0.1 EU per 1 μg of the protein by the LAL method.

    Form

    Lyophilized

• Background

  • Background

    dCas9-VP64 refers to the dCas9 protein (#PDB: 5Y36_A) fused with the VP64 transcription activation domain, which is used in CRISPR-based gene activation. Comprising a deactivated Cas9 (dCas9) protein and VP64 transcriptional activator, it precisely targets gene promoters, enhancing gene expression without altering the DNA sequence. dCas9-VP64 offers a versatile tool for studying gene function, disease mechanisms, and potential therapeutic interventions.
    dCas9 protein is an endonuclease-inactive version of Cas9 (CRISPR associated protein 9). dCas9 lacks endonuclease activity so that it binds but does not cleave cognate DNA, which can be used to localize transcriptional activators or repressors to specific DNA sequences in order to control transcription.

    Synonyms

    CRISPR-associated endonuclease Cas9/Csn1, SpCas9, SpyCas9

  • Sequence Note

    Met1-Asp1368

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  SDS-PAGE analysis of recombinant dCas9–VP64.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.