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Species of Origin
Enterovirus A71
Expression System
Escherichia coli
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Affinity Tag
His Tag (C-term)
Storage Buffer
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
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Purity
>95% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 33.76 kDa.
The protein migrates as 35 kDa under reducing condition (SDS-PAGE analysis). -
Form
Lyophilized
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Specifications
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Background
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Background
Enterovirus A71 (EV-A71), a positive-strand RNA virus of the Picornaviridae family, is a persisting global concern. EV-A71 is primarily transmitted through the fecal-oral route, causing hand, foot, and mouth disease (HFMD), severe central nervous system complications, neural damage, and even life-threatening. VP1 is a structural capsid protein consisting of 297 amino acids, promoting infection by binding to specific receptors on the surface of host cells, including P-selectin glycoprotein ligand 1 (PSGL-1), scavenger receptor B2 (SCARB2), annexin II protein, sialylated glycan, and heparan sulfate.
Synonyms
P1D, Virion protein 1
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Uniprot ID
#BDI13007.1 (GeneBank ID)
Sequence Note
Gly1-Ser297
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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Publication
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Datasheet & Documents
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Disclaimer:For Research Use or Further Manufacturing Only.