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Leadgene Leadgene
HRV 3C Protease ELISA Kit
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Catalog Number

LDG00017E

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  • Overview

    Description

    HRV 3C Protease is a recombinant cysteine protease from human rhinovirus 3C. It can recognize and cleave the sequence: : Leu-Glu-Val-Leu-Phe-Gln↓Gly-Pro (LEVLFQ↓GP). This protease is used to remove fusion tags from recombinant proteins with the specific cleavage site. Therefore, detection of HRV 3C Protease is important for evaluating the level of HRV 3C Protease in samples. Leadgene® HRV 3C Protease ELISA kit is an enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of HRV 3C Protease level in cleavage sample solution. The HRV 3C Protease ELISA kit is for research use only (RUO). Not suitable for use in diagnostic or therapeutic procedures.

    Test Principle

    HRV 3C Protease ELISA kit is used to detect HRV 3C Protease in samples by sandwich ELISA method. This assay uses microplate pre-coated with mouse anti-HRV 3C Protease monoclonal antibody to the solid phase. HRV 3C Protease in the samples conjugates on solid phase and then react with the HRP conjugated mouse anti-HRV 3C Protease monoclonal antibody. Subsequent wash steps will residual unbound antibody. After incubation with substrate solution, the reaction is determined by the absorbance at 450 nm. Quantification of HRV 3C Protease level is accomplished by comparing the absorbance with standard curve.

  • Specifications
    • Reactivity

      Human rhinovirus

      Application

      Sandwich ELISA analysis

    • Detection Method

      Colorimetric

      Sensitivity

      Limit of detection (LoD): 0.057 ng/mL
      Limit of quantification (LoQ): 0.19 ng/mL

    • Specificity

      HRV 3C Protease

      Assay Range

      1.5625-100 ng/mL

    • Sample Type

      Samples contain HRV 3C Protease

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at 2-8°C for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8℃ for unopened product.

      Please refer to product manual for storage constructions.

  • Image
    Typical data The following data are for demonstration only

    Typical data
    The following data are for demonstration only

    Reagents provided and reconstitution

    Reagents provided and reconstitution

    An example of orientation of standards, blanks and samples in the stripwells microplate

    An example of orientation of standards, blanks and samples in the stripwells microplate

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  • Datasheet & Documents
    Datasheet
    MSDS
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Disclaimer:For Research Use or Further Manufacturing Only.

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