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Leadgene Leadgene
Human Activin B, His Tag, E. coli
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Catalog Number

LDG064PHE

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  • Specifications
    • Species of Origin

      Human

      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 13.75 kDa.
      The protein migrates as 14 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce hemoglobin expression in K562 cells. The ED₅₀ for this effect is <0.7 ng/mL. The specific activity of recombinant human Activin B is > 1.5 x 10⁶ IU/mg.

      Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Activin B is the cytokine of the TGF-beta family, which is a 13 kDa protein containing 115 amino acid residues. Activin B has the wide variety of biological function including stem cell differentiation, inflammation, bone remodeling and neural development. They also have a role in regulation of FSH secretion. Activin B induces the phosphorylation of Smad which binds the smad binding element.

      Synonyms

      Inhibin beta B chain, Activin beta-B chain, INHBB

    • Uniprot ID

      #P09529

      Sequence Note

      Gly293-Ala407

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    Human Activin B, His Tag, E. coli (LDG064PHE) induced hemoglobin expression in K562 cells, with the ED50 at 0.651 ng/mL.

    Human Activin B, His Tag, E. coli (LDG064PHE) induced hemoglobin expression in K562 cells, with the ED50 at 0.651 ng/mL.

    SDS-PAGE analysis of recombinant human Activin B.

    SDS-PAGE analysis of recombinant human Activin B.

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  • Datasheet & Documents
    MSDS
    Manual
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