Human BMP-10, His Tag, E. coli

Catalog Number

LDG108PHE

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression System

    Escherichia coli

  • Affinity Tag

    His Tag (C-term)

    Storage Buffer

    Lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate and 0.2 M NaCl, pH 3.5.

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 13.10 kDa.
    The protein migrates as 16 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by its ability to induce alkaline phosphatase production by ATDC5 cells. The ED₅₀ for this effect is 1.7-2.1 ng/mL.

    Endotoxin Level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    Bone Morphogenetic Protein-10 (BMP-10) is an extracellular multifunctional signaling cytokine that is also a member of the TGFβ family. BMP-10 can bind with TGFβ receptor and is involved in SMAD protein signal transduction. The functions related to bone generation can induce bone and cartilage formation. Different from other family members, BMP-10 is a novel protein involved in the heart's trabeculation.

    Synonyms

    Bone morphogenetic protein 10

  • Uniprot ID

    #O95393

    Sequence Note

    Asn317-Arg424

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in 4 mM HCl to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  SDS-PAGE analysis of recombinant human BMP-10.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.