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Species of Origin
Human
Expression System
Escherichia coli
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Affinity Tag
His Tag (C-term)
Storage Buffer
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
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Purity
>98% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 41.43 kDa.
The protein migrates as 40 kDa under reducing condition (SDS-PAGE analysis). -
Endotoxin Level
<0.1 EU per 1 μg of the protein by the LAL method.
Form
Lyophilized
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Specifications
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Background
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Background
Chitinases and non-enzymatic chitinase-like proteins (CLPs, can bind chitin but cannot digest) belong to Glycoside hydrolase family 18. Human CHI3L1, YKL-40, is a CLP and a 42 kDa protein with 383 amino acid residues. The complex (CHI3L1, IL-13R alpha2 and IL-13) regulate downstream signal transduction pathway related to inflammation, proliferation and metastasis.
Synonyms
39 kDa synovial protein, Cartilage glycoprotein 39, CGP-39, GP-39, hCGP-39, YKL-40, Chitinase-3-like protein 1
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Uniprot ID
#P36222
Sequence Note
Tyr22-Thr383
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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Image
![SDS-PAGE analysis of recombinant human CHI3L1.]()
SDS-PAGE analysis of recombinant human CHI3L1.
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Review
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Publication
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Datasheet & Documents
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Disclaimer：For Research Use or Further Manufacturing Only.
