Human CXCL12 (aa 24-88), His Tag, E. coli

Catalog Number

LDG053PHE

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression System

    Escherichia coli

  • Affinity Tag

    His Tag (C-term)

    Storage Buffer

    Lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 4.5.

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 8.55 kDa.
    The protein migrates as 10 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by its ability to chemoattract BaF3 cells transfected with human CXCR4. The ED₅₀ for this effect is <0.5 ng/mL.

    Endotoxin Level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    C-X-C motif chemokine 12 (CXCL12) also named stromal cell-derived factor 1 (SDF-1), which is a chemokine of the intercrine alpha family. CXCL12 is a 19.5kDa protein containing 167 amino acid residues. The function of CXCL12 include the hematopoiesis, angiogenesis embryogenesis, inflammation and angiogenesis. CXCL12 activated by the IL-1, TNF or lipopolysaccharide and induce the immune response in leukocytes.

    Synonyms

    Stromal cell-derived factor 1, SDF-1, C-X-C motif chemokine 12, Intercrine reduced in hepatomas, IRH, hIRH, Pre-B cell growth-stimulating factor, PBSF, SDF-1-beta, 3-72, SDF-1-alpha, 3-67

  • Uniprot ID

    #P48061

    Sequence Note

    Val24-Asn88

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  SDS-PAGE analysis of recombinant human CXCL12 (aa 24-88).

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.