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Leadgene Leadgene
Human CXCL9, His Tag, E. coli
Catalog Number

LDG160PHE

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  • Specifications
    • Species of Origin

      Human

      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (N-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 12.53 kDa.
      The protein migrates as 18 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to chemoattract human THP1 cells using a concentration range of 50-200 ng/mL.

      Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      C-X-C motif chemokine 9 (CXCL9) also named monokine induced by gamma interferon (MIG), which is a chemokine of the intercrine alpha family. CXCL9 is a 11.4kDa protein containing 103 amino acid residues. CXCL9 controls the immune cells by binding the CXCR3 which is including the cell migration and activation. During inflammation, CXCL9 is a chemotaxis for lymphocyte and macrophages. CXCL9 is participated in the process of tumor proliferation and metastasis.

      Synonyms

      Gamma-interferon-induced monokine, Monokine induced by interferon-gamma, HuMIG, MIG, Small-inducible cytokine B9, C-X-C motif chemokine 9

    • Uniprot ID

      # Q07325 1

      Sequence Note

      Thr21-Thr125

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant human CXCL9.

    SDS-PAGE analysis of recombinant human CXCL9.

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  • Datasheet & Documents
    MSDS
    Manual
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