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Species of Origin
Human
Expression System
Escherichia coli
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Affinity Tag
His Tag (N-term)
Storage Buffer
Lyophilized from a 0.2 µm filtered solution of PBS with 7 mM MgSO₄, pH 7.2
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Purity
>98% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 47.85 kDa.
The protein migrates as 55 kDa under reducing condition (SDS-PAGE analysis). -
Endotoxin Level
<0.1 EU per 1 μg of the protein by the LAL method.
Form
Lyophilized
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Specifications
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Background
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Background
Enolase 1 (ENO1) also named alpha-enolase which is a glycolytic enzyme. It could convert the NAD+ to NADH by producing two ATP. ENO1 is a homodimer which is composed by two isozymes of enolase (2 α, 2 ν, or 2 β). It also plays an important role in cancer model which can promote tumor cell proliferation and migration by the PI3K signaling pathway. ENO1 is a biomarker of prognostic and diagnostic cancer.
Synonyms
Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding protein, Phosphopyruvate hydratase, Plasminogen-binding protein
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Uniprot ID
# P06733 2
Sequence Note
Ser2-Lys434
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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Image
![SDS-PAGE analysis of recombinant human ENO1.]()
SDS-PAGE analysis of recombinant human ENO1.
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Publication
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Datasheet & Documents
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Disclaimer：For Research Use or Further Manufacturing Only.
