Human FGF-1, His Tag, E. coli

Catalog Number

LDG067PHE

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression System

    Escherichia coli

  • Affinity Tag

    His Tag (C-term)

    Storage Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 16.77 kDa.
    The protein migrates as 18kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <0.3 ng/mL. The specific activity of recombinant human FGF-1 is > 1 x 10⁶ IU/mg.

    Endotoxin Level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    Fibroblast Growth Factors-1 (FGF-1) is a Growth Factors of mitogenic peptides which is a 15 kDa protein containing 139 amino acid residues. Fibroblast Growth Factors-1 is secreted by the macrophage which induce endothelial cell proliferation and angiogenesis. During wound injury, fibroblast Growth Factors-1 helps the tissue remodeling. When the fibroblast Growth Factors-1 binds with the FGFR on the cell membrane which is endocytosed by FGFR and induces the cell cycle.

    Synonyms

    Acidic fibroblast Growth Factors , aFGF, Endothelial cell Growth Factors , ECGF, Heparin-binding Growth Factors 1 , HBGF-1, Fibroblast Growth Factors 1

  • Uniprot ID

    #P05230

    Sequence Note

    Phe16-Asp155

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  SDS-PAGE analysis of recombinant human FGF-1.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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