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Leadgene Leadgene
Human FGF-12, His Tag, E. coli
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Catalog Number

LDG077PHE

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  • Specifications
    • Species of Origin

      Human

      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 21.23 kDa.
      The protein migrates as 21 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <2 ng/mL.

      Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Fibroblast Growth Factors-12 (FGF-12) is a 20.4 kDa member of the fibroblast Growth Factors with 181 amino acid residues. FGF-12 is mainly expressed from inhibitory neurons, oligodendrocyte precursor cells, excitatory neurons, cardiomyocytes. Involved in nervous system development, function, and positive regulation of voltage-gated sodium channel activity. FGF-12 function in the development of the central and peripheral nervous systems, connective tissue of the skeleton, and the myocardia of the heart.

      Synonyms

      Fibroblast Growth Factors homologous factor 1, FHF-1, Fibroblast Growth Factors 12

    • Sequence Note

      #NP_004104.3 Met1-Thr181

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    Human FGF-12, His Tag, E. coli (LDG077PHE) induced 3T3 cell proliferation, with the ED50 at 1.61 ng/mL.

    Human FGF-12, His Tag, E. coli (LDG077PHE) induced 3T3 cell proliferation, with the ED50 at 1.61 ng/mL.

    SDS-PAGE analysis of recombinant human FGF-12.

    SDS-PAGE analysis of recombinant human FGF-12.

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  • Datasheet & Documents
    MSDS
    Manual
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