Human FGF-23, His Tag, E. coli

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Catalog Number

LDG087PHE

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression System

    Escherichia coli

  • Affinity Tag

    His Tag (C-term)

    Storage Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 26.27 kDa.
    The protein migrates as 26 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by its ability to induce proliferation in BaF3 cells transfected with human FGFRIIIc. The ED₅₀ for this effect is <0.3 μg/mL.

    Endotoxin Level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    Fibroblast Growth Factors-23 (FGF-23) is a 28 kDa member of the fibroblast Growth Factors with 251 amino acid residues. FGF-23 is expressed from brain, hepatic stellate cells, cone photoreceptor cells, early spermatids. FGF-23 involved phosphate metabolism and vitamin D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization.

    Synonyms

    Fibroblast Growth Factors 23 N-terminal peptide, Fibroblast Growth Factors 23 C-terminal peptide, Phosphatonin, Tumor-derived hypophosphatemia-inducing factor

  • Uniprot ID

    #Q9GZV9

    Sequence Note

    Tyr25-Ile251

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  Human FGF-23, His Tag, E. coli (LDG087PHE) induced proliferation in BaF3 cells transfected with human FGFRIIIc, with the ED50 at 0.26 μg/mL.

  SDS-PAGE analysis of recombinant human FGF-23.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.