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Leadgene Leadgene
Human GM-CSF, His Tag, E. coli
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Catalog Number

LDG044PHE

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  • Specifications
    • Species of Origin

      Human

      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (N-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 15.4 kDa.
      The protein migrates as 15 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce TF-1 cells proliferation. The ED₅₀ for this effect is <80 pg/mL. The specific activity of recombinant human GM-CSF is approximately >1 x 10⁷ IU/mg.

      Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Granulocyte-macrophage colony-stimulating factor (GM-CSF) was first identified as a Growth Factors due to its ability to induce proliferation and differentiation of bone marrow progenitors into granulocytes and macrophages. GM-CSF is produced by multiple cell types including activated T cells, B cells, macrophages, endothelial cells and fibroblasts upon receiving immune stimuli. GM-CSF stimulates stem cells to produce granulocytes and monocytes functions as a cytokine.

      Synonyms

      Granulocyte-macrophage colony-stimulating factor, Colony-stimulating factor , CSF, Molgramostin, Sargramostim

    • Uniprot ID

      #P04141

      Sequence Note

      Ala18-Glu144

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    Human GM-CSF, His Tag, E. coli (LDG044PHE) induced TF-1 cell proliferation, with the ED50 at 54.7 pg/mL.

    Human GM-CSF, His Tag, E. coli (LDG044PHE) induced TF-1 cell proliferation, with the ED50 at 54.7 pg/mL.

    SDS-PAGE analysis of recombinant human GM-CSF.

    SDS-PAGE analysis of recombinant human GM-CSF.

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  • Datasheet & Documents
    MSDS
    Manual
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