Human GM-CSF, His Tag, E. coli

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Catalog Number

LDG044PHE

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression System

    Escherichia coli

  • Affinity Tag

    His Tag (N-term)

    Storage Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 15.4 kDa.
    The protein migrates as 15 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by its ability to induce TF-1 cells proliferation. The ED₅₀ for this effect is <80 pg/mL. The specific activity of recombinant human GM-CSF is approximately >1 x 10⁷ IU/mg.

    Endotoxin Level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    Granulocyte-macrophage colony-stimulating factor (GM-CSF) was first identified as a Growth Factors due to its ability to induce proliferation and differentiation of bone marrow progenitors into granulocytes and macrophages. GM-CSF is produced by multiple cell types including activated T cells, B cells, macrophages, endothelial cells and fibroblasts upon receiving immune stimuli. GM-CSF stimulates stem cells to produce granulocytes and monocytes functions as a cytokine.

    Synonyms

    Granulocyte-macrophage colony-stimulating factor, Colony-stimulating factor , CSF, Molgramostin, Sargramostim

  • Uniprot ID

    #P04141

    Sequence Note

    Ala18-Glu144

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  Human GM-CSF, His Tag, E. coli (LDG044PHE) induced TF-1 cell proliferation, with the ED50 at 54.7 pg/mL.

  SDS-PAGE analysis of recombinant human GM-CSF.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.