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Leadgene Leadgene
Human IFN Gamma ELISA Kit
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Catalog Number

LDG00023E

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  • Overview

    Description

    The cytokine IFN gamma could protect cells from viral infections and belongs to the family of interferons. A lot of studies have shown that IFN gamma secreted by antigen triggered cell types, including T cells, naive CD4+ T cells, macrophages, dendritic cells, and B cells. IFN gamma plays an important role to trigger the macrophage act against a diverse group of microbial targets, and the pleiotropic molecule associated with antiproliferative, pro-apoptotic and antitumor mechanisms. Based on the effector cytokine considered as a major effector of immunity, it has been used in the treatment of several diseases.

    Test Principle

    Human IFN Gamma ELISA kit is used to detect IFN Gamma in samples by sandwich ELISA method. This assay uses microplate pre-coated with mouse anti-human IFN gamma monoclonal antibody to the solid phase. Human IFN Gamma in the sample conjugates on solid phase and then react with the HRP conjugated mouse anti-human IFN gamma monoclonal antibody. Subsequent wash steps will remove residual unbound antibody. After incubation with substrate solution, the reaction is determined by the absorbance at 450 nm. Quantification of nuclease level is accomplished by comparing the absorbance with standard curve.

  • Specifications
    • Reactivity

      Human

      Application

      Sandwich ELISA analysis

    • Sensitivity

      0.00606 ng/mL

      Specificity

      Human IFN Gamma

    • Assay Range

      0.015625 ng/mL-1 ng/mL

      Sample Type

      Human Serum / Human Plasma

    • Recovery

      Human Serum: 90-109%
      Human Plasma: 8X dilution: 83%

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at 2-8°C for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8℃ for unopened product.

      Please refer to product manual for storage constructions.

  • Image
    Typical data The following data are for demonstration only.

    Typical data
    The following data are for demonstration only.

    An example of orientation of standards, blanks and samples in the stripwells microplate

    An example of orientation of standards, blanks and samples in the stripwells microplate

    Reagents provided and reconstitution

    Reagents provided and reconstitution

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  • Datasheet & Documents
    Datasheet
    MSDS
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Disclaimer:For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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