Human IL-30, His Tag, E. coli

Catalog Number

LDG034PHE

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression System

    Escherichia coli

  • Affinity Tag

    His Tag (C-term)

    Storage Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 25.47 kDa.
    The protein migrates as 28 kDa under reducing condition (SDS-PAGE analysis).

  • Endotoxin Level

    <0.1 EU per 1 μg of the protein by the LAL method.

    Form

    Lyophilized

• Background

  • Background

    Interleukin 30 (IL-30) is a 27 kDa cytokine with 216 amino acid residues. IL-30, also known as IL-27A, forms a complex named IL-27 with Ebi3 and is secreted from cells of myeloid origin, such as monocytes, macrophages, and dendritic cells. IL-30 /Ebi3 complex mediates T-cell immunity, promoting Th1 differentiation, inhibiting Th2 differentiation, and inhibiting Th17 differentiation. IL-30 also can be secreted independently of Ebi3 and modulate bacteria-induced oxidative burst and cytokine production.

    Synonyms

    IL-27 subunit alpha, IL-27-A, Interleukin-30, p28

  • Uniprot ID

    #Q8NEV9

    Sequence Note

    Phe 29-Pro 243

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  SDS-PAGE analysis of recombinant human IL-30.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.