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Leadgene Leadgene
Human IL-6 ELISA Kit
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Catalog Number

LDG00025E

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  • Overview

    Description

    Interleukin-6 (IL-6) is a pleiotropic, 22-28 kDa cytokine which plays fundamental role in the acute phase response, inflammation, bone metabolism, lymphocyte differentiation and cancer progression. Deregulation of IL-6 production was also found in several diseases, including rheumatoid arthritis, Alzheimer’s disease, autoimmune deficiency disease and different types of cancer.

    Test Principle

    Human IL-6 ELISA kit is used to detect IL-6 in samples by sandwich ELISA method. This assay uses microplate pre-coated with mouse anti-human IL-6 monoclonal antibody to the solid phase. Human IL-6 in the sample conjugates on solid phase and then react with the HRP conjugated mouse anti-human IL-6 monoclonal antibody. Subsequent wash steps will remove residual unbound antibody. After incubation with substrate solution, the reaction is determined by the absorbance at 450 nm. Quantification of nuclease level is accomplished by comparing the absorbance with standard curve.

  • Specifications
    • Reactivity

      Human

      Application

      Sandwich ELISA analysis

    • Sensitivity

      0.0042 ng/mL

      Specificity

      Human IL-6

    • Assay Range

      0.015625 ng/mL-1 ng/mL

      Sample Type

      Human Serum / Human Plasma

    • Recovery

      Serum
      Stock: 73.4%
      2x dilution: 94.4%
      4x dilution: 103.6%
      8x dilution: 104.4%

      Plasma
      Stock: 82.6%
      2x dilution: 84%
      4x dilution: 90.2%
      8x dilution: 93.6%

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at 2-8°C for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8℃ for unopened product.

      Please refer to product manual for storage constructions.

  • Image
    Typical data The following data are for demonstration only.

    Typical data
    The following data are for demonstration only.

    An example of orientation of standards, blanks and samples in the stripwells microplate

    An example of orientation of standards, blanks and samples in the stripwells microplate

    Reagents provided and reconstitution

    Reagents provided and reconstitution

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  • Datasheet & Documents
    Datasheet
    MSDS
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Disclaimer:For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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