Human TGF Alpha, His Tag, E. coli

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Catalog Number

LDG090PHE

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression System

    Escherichia coli

  • Affinity Tag

    His Tag (C-term)

    Storage Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 6.49 kDa.
    The protein migrates as 7 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by its ability to induce 3T3 cells proliferation. The ED₅₀ for this effect is <0.2 ng/mL. The specific activity of recombinant human TGF alpha is > 5 x 10⁶ IU/mg.

    Endotoxin Level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    Transforming Growth Factors alpha (TGF-α) is a 5.68 kDa member of the epidermal Growth Factors with 51 amino acid residues. TGF-α is mainly expressed from brain, skin, epithelial cell (pancreatic endocrine cells, urothelial cells, oligodendrocyte precursor cells, etc.). TGF-α is a regulator of cell proliferation and differentiation via bind to the EGFR and to act synergistically with TGF β. TGF-α also associates with myriad forms of cancer.

    Synonyms

    EGF-like TGF, ETGF, TGF type 1, Protransforming Growth Factors alpha

  • Uniprot ID

    #P01135

    Sequence Note

    Trp40-Ala89

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  Human TGF Alpha, His Tag, E. coli (LDG090PHE) induced 3T3 cell proliferation, with the ED50 at 0.19 ng/mL.

  SDS-PAGE analysis of recombinant human TGF Alpha.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.