Human TGF Beta 3, His Tag, E. coli

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Catalog Number

LDG152PHE

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression System

    Escherichia coli

  • Affinity Tag

    His Tag (C-term)

    Storage Buffer

    Lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate and 0.2 M NaCl, pH 4.5.

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 13.66 kDa.
    The protein migrates as 13 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by its ability to inhibit IL-4-induce proliferation in HT-2 cells. The ED₅₀ for this effect is < 50 pg/mL. The specific activity of recombinant human TGF Beta 3 is approximately 2 x 10⁷ IU/mg.

    Endotoxin Level

    <0.1 EU per 1 μg of the protein by the LAL method.

  • Form

    Lyophilized

• Background

  • Background

    Transforming Growth Factors-Beta 3 (TGF-beta 3) is belonging to the TGF-β superfamily TGF-β3 is 86%, 91% similar to TGF-β1 and TGF-β2. TGF-beta 3 is a 12.8 kDa protein containing 412 amino acids, which could inhibitor of DNA synthesis, increases cellular proliferation, essential mediator of EMT in cardiac morphogenesis, and up-regulated by milk stasis and induces apoptosis in mammary gland epithelium during involution.

    Synonyms

    Transforming Growth Factors beta-3 proprotein, Latency-associated peptide, LAP, Transforming Growth Factors beta-3, TGF-beta-3

  • Uniprot ID

    # P10600 1

    Sequence Note

    Ala301-Ser412

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  Human TGF Beta 3, His Tag, E. coli (LDG152PHE) inhibited IL-4-induced HT-2 cell proliferation, with the ED50 at 43 pg/mL.

  SDS-PAGE analysis of recombinant human TGF beta 3.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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