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Leadgene Leadgene
Human TRAIL, His Tag, E. coli
Catalog Number

LDG121PHE

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  • Specifications
    • Species of Origin

      Human

      Expression System

      Escherichia coli

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

    • Purity

      >98% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 20.33 kDa.
      The protein migrates as 18 kDa under reducing condition (SDS-PAGE analysis).

    • Activity

      Measure by its ability to induce cytotoxicity in L929 cells in the presence of actinomycin D. The ED₅₀ for this effect is 10.4-15.4 ng/mL.

      Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

    • Form

      Lyophilized

  • Background
    • Background

      Tumor necrosis factor‑associated apoptosis‑inducing ligand (TRAIL) is a type II membrane protein of the tumor necrosis factor (TNF) family members, moreover, expressed in many adult tissues including the thymus, prostate, colon, ovary and lung. TRAIL is a 19kDa protein containing 281 residues. TRAIL to induce apoptosis in human breast carcinoma cells (MCF7) and human epitheloid carcinoma (HeLa) cell lines, by activate two death receptors of DR4 and DR5 or two decoy receptors DcR1 and DcR2.

      Synonyms

      TNF-related apoptosis-inducing ligand , TRAIL, Apo-2 ligand, Apo-2L, CD253, Tumor necrosis factor ligand superfamily member 10, TNFL10

    • Uniprot ID

      #P50591

      Sequence Note

      Arg114-Gly281

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:

      • -20°C for 12 months in lyophilized state from date of receipt.
      • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant human TRAIL.

    SDS-PAGE analysis of recombinant human TRAIL.

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  • Datasheet & Documents
    MSDS
    Manual
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