LeadGMP® Human IFN Gamma Protein, His Tag, E. coli

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Catalog Number

LDG013PHE-GMP

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression System

    Escherichia coli

  • Affinity Tag

    His Tag (C-term)

    Storage Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 8.0.

  • Purity

    >97% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 17.7 kDa.
    The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by its ability to induce cytotoxicity in HT29 cells. The ED₅₀ for this effect is <1 ng/mL. The specific activity of recombinant human IFN gamma is approximately >1 x 10⁷ IU/mg, which is calibrated against the human IFN Gamma WHO Reference Material (NIBSC code: 87/586).

    Endotoxin Level

    <0.05 EU per 1 µg of the protein by the LAL method.

  • Mycoplasma

    Not detected

    Form

    Lyophilized

• Background

  • Background

    The cytokine IFN gamma could protect cells from viral infections and belongs to the family of interferons. A lot of studies have shown that IFN gamma secreted by antigen triggered cell types, including T cells, naive CD4+ T cells, macrophages, dendritic cells, and B cells. IFN gamma plays an important role to trigger the macrophage act against a diverse group of microbial targets, and the pleiotropic molecule associated with antiproliferative, pro-apoptotic and antitumor mechanisms. Based on the effector cytokine considered as a major effector of immunity, it has been used in the treatment of several diseases.

    Synonyms

    Interferon gamma, Immune interferon

  • Uniprot ID

    P01579

    Sequence Note

    Gln24-Gln166

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 0.5 mg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

    Manufacturing Specifications

    LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:

    • Animal-free reagent and laboratory
    • Manufactured and tested under GMP guideline
    • Testing and traceability of raw material
    • Records of the maintenance and equipment calibration
    • Personnel training records
    • Batch-to-batch consistency
    • Documentation of QA control and process changes
    • Manufactured and tested under an ISO 13485:2016 certified quality management system
    • Stability monitor of product shelf-life

• Image

  LeadGMP® Human IFN Gamma Protein, His Tag, E. coli (LDG013PHE-GMP) induces cytotoxicity in HT29 cells, with the ED50 <1.0 ng/mL.

  SDS-PAGE analysis of LeadGMP® IFN gamma, Human

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.