LeadGMP® Human IL-18 Protein, Tag Free, E. coli

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Catalog Number

LDG025PHE-GMP

Datasheet

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• Specifications

  • Species of Origin

    Human

    Expression System

    Escherichia coli

  • Affinity Tag

    Tag Free

    Storage Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

  • Purity

    >97% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 18.2 kDa.
    The protein migrates as 15 kDa under reducing condition (SDS-PAGE analysis).

  • Activity

    Measure by its ability to induce IFN gamma secretion in KG-1 cells. The ED₅₀ for this effect is <6 ng/mL. The specific activity of recombinant LeadGMP® human IL-18 is > 1 x 10⁷ IU/mg, which is calibrated against the human IL-18 WHO Reference Reagent (NIBSC code: 03/200).

    Endotoxin Level

    <0.05 EU per 1 µg of the protein by the LAL method.

  • Mycoplasma

    Not detected

    Form

    Lyophilized

• Background

  • Background

    Interleukin-18 (IL-18) belongs to the IL-1 superfamily and is constitutively produced by macrophages, dendritic cells (DCs) and other cells. IL-18 binds to the receptor of IL-18 (IL-18R) and initiate the recruitment and heterodimerization of the IL-18RAcP, leading to downstream activation of NF-κB. After stimulation with IL-18, multiple cytokines including IFN-γ, IL-13, IL-4, IL-8, and GM-CSF and both Th1 and Th2 lymphokines could be produced by different cells. As an immunoregulaory cytokine, IL-18 can promote development of T helper 1 (Th1) cells, which maximizes the production of IFN by synergistically stimulating to mature Th1 effectors in combination with IL-12. On the contrary, it inhibits the production of the anti-inflammatory cytokine IL-10. In addition, IL-18 exhibits multiple proinflammatory functions, such as increases in cell adhesion molecules, nitric oxide synthesis, and chemokine production.

    Synonyms

    Interleukin-18, Iboctadekin, Interferon gamma-inducing factor, IFN-gamma-inducing factor, Interleukin-1 gamma , IL-1 gamma

  • Uniprot ID

    Q14116

    Sequence Note

    Tyr37-Asp193

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 0.5 mg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

    Manufacturing Specifications

    LeadGMP® recombinant proteins are manufactured in ISO 13485:2016 and GMP certified facility. The processes include:

    • Animal-free reagent and laboratory
    • Manufactured and tested under GMP guideline
    • Testing and traceability of raw material
    • Records of the maintenance and equipment calibration
    • Personnel training records
    • Batch-to-batch consistency
    • Documentation of QA control and process changes
    • Manufactured and tested under an ISO 13485:2016 certified quality management system
    • Stability monitor of product shelf-life

• Image

  LeadGMP® Human IL-18 Protein, Tag Free, E. coli (LDG025PHE-GMP) induces IFN gamma secretion in KG-1 cells, with the ED50 < 6 ng/mL.

  SDS-PAGE analysis of LeadGMP® IL-18, Human

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.