M-MLV Reverse Transcriptase

Catalog Number

LDG0006RF

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• Overview

  Description

  Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase is an RNA-dependent DNA polymerase that synthesizes the first strand of complementary cDNA from a single-stranded RNA template with hybridized primer. This kit features high activity formulation of M-MLV RT and 5X reverse transcription buffer which are capable of full-length cDNA synthesis and high cDNA yields.

  Product Note

  5X M-MLV Reverse Transcriptase Buffer: 250 mM Tris-HCl (pH 8.3), 15 mM MgCl₂, 375 mM KCl, and 50 mM DTT.
  After the reaction is complete, M-MLV RTase can be inactivated by incubation at 65°C for 20 minutes

  Components

  Package	Items	Quantity
  20,000 U	M-MLV Reverse Transcriptase (200 U/μL)	1 vial (20,000 U)
  	5× M-MLV Reverse Transcriptase Reaction Buffer	1 vial (1 mL)
  50,000 U	M-MLV Reverse Transcriptase (200 U/μL)	1 vial (50,000 U)
  	5× M-MLV Reverse Transcriptase Reaction Buffer	1 vial (1 mL)

  • 20,000 U

    Items

    M-MLV Reverse Transcriptase (200 U/μL)

    Quantity

    1 vial (20,000 U)

    Items

    5× M-MLV Reverse Transcriptase Reaction Buffer

    Quantity

    1 vial (1 mL)

  • 50,000 U

    Items

    M-MLV Reverse Transcriptase (200 U/μL)

    Quantity

    1 vial (50,000 U)

    Items

    5× M-MLV Reverse Transcriptase Reaction Buffer

    Quantity

    1 vial (1 mL)

• Specifications

  • Expression System

    Escherichia coli

    Application

    Reverse transcription, RT-PCR

  • Concentration

    200 U/uL

    Storage Buffer

    M-MLV Reverse Transcriptase is supplied in 20 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.25 mM EDTA, 0.01% NP-40 (v/v), 2.5 mM DTT and 50% glycerol (v/v).

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Unit Definition

    One unit is defined as the amount of the enzyme incorporates 1 nmol of dTTP into acidinsoluble product in 10 minutes at 37°C.

  • Form

    Liquid

• Instruction

  • Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    Stability & Storage

    This product is stable after storage at:

    • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

    Avoid repeated freeze/thaw cycles.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.