Description
Affinity purified anti-HA antibodies, developed in mouse, were conjugated to NHS-sepharose. It is an efficient technique for isolating recombinant proteins or other proteins. The HA epitope system relies on the peptide (N-Tyr-Pro-Tyr-Asp-Val-Pro-Asp-Tyr-Ala-C), which is able to react with N- and C- terminal HA tagged fusion proteins and may be used for the immunoprecipitation or immune affinity purification.
The purified antibody is immobilized at 3-5 mg antibody per mL 50% slurry and this kit allows a rapid and efficient affinity purification of active HA fusion proteins. The affinity resin allows an efficient binding of HA fusion proteins without the need for preliminary steps and calibrations. The affinity bound HA fusion proteins can be efficiently eluted from the resin by acid condition. The eluted proteins can be used for characteristic analysis.
Product Note
The optimal protein concentration of the cell lysate should be determined by the end user.
