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Leadgene Leadgene
Mouse CD276, His Tag, HEK293
Catalog Number

LDG039PMM

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  • Specifications
    • Species of Origin

      Mouse

      Expression System

      HEK293

    • Affinity Tag

      His Tag (C-term)

      Storage Buffer

      Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

    • Purity

      >90% as determined by SDS-PAGE analysis.

      Molecular weight

      The protein has a calculated MW of 24.32 kDa.
      The protein migrates as 30-38 kDa under reducing condition (SDS-PAGE analysis).

    • Endotoxin Level

      <0.1 EU per 1 μg of the protein by the LAL method.

      Mycoplasma

      Not detected.

    • Form

      Lyophilized.

  • Background
    • Background

      B7-H3 (CD276) is an immune checkpoint molecule that modulates T cell responses. It is involved in immune evasion by tumors and is a target for cancer immunotherapy.

      Synonyms

      CD276 antigen, B7 homolog 3, B7-H3, Costimulatory molecule, CD276

    • Uniprot ID

      Q8VE98

      Sequence Note

      Val 29 - Phe 244

  • Instruction
    • Reconstitution

      It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

      Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    • Stability & Storage

      This product is stable after storage at:
      ● -20°C for 12 months in lyophilized state from date of receipt.
      ● -20°C or -80°C for 1 month under sterile conditions after reconstitution.
      Avoid repeated freeze/thaw cycles.

  • Image
    SDS-PAGE analysis of recombinant mouse CD276.

    SDS-PAGE analysis of recombinant mouse CD276.

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  • Datasheet & Documents
    MSDS
    Manual
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