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Species of Origin
Mouse
Expression System
Escherichia coli
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Affinity Tag
His Tag (C-term)
Storage Buffer
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
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Purity
>98% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 17.81 kDa.
The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis). -
Activity
Measure by its ability to inhibit IL-36 gamma-induced IL-6 secretion in 3T3 cells. The ED₅₀ for this effect is <2 µg/mL.
Endotoxin Level
<0.1 EU per 1 μg of the protein by the LAL method.
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Form
Lyophilized
![Mouse IL-36RA, His Tag, E. coli](https://www.leadgenebio.com/upload/website/product/model/950/images/LDG059PMEPAGE01.webp)
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Specifications
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Background
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Background
Interleukin 36 receptor antagonist (IL-36RA) is a 17.21 kDa cytokine with 155 amino acid residues and a member of the IL-36 family. It is mainly secreted from the stomach, lungs, and kidneys. IL-36RA binds to the interleukin-36 receptor and plays a critical role in the anti-inflammatory effect by mediating MAPK and NF-κB signaling pathways. IL-36RA inhibits proinflammatory effect via suppressing production of IL-6, IL-17, and interferon-gamma and promoting production of IL-4.
Synonyms
Interleukin-36 receptor antagonist protein, IL-36Ra, Interleukin-1 HY1, IL-1HY1, Interleukin-1 delta, IL-1 delta, Interleukin-1 family member 5, IL-1F5, Interleukin-1 homolog 3, IL-1H3, Interleukin-1-like protein 1, IL-1L1
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Uniprot ID
#Q9QYY1
Sequence Note
Met2-Asp156
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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Image
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Review
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Publication
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Datasheet & Documents
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Disclaimer:For Research Use or Further Manufacturing Only.