Mouse Midkine, His Tag, E. coli

Catalog Number

LDG088PME

Datasheet

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• Specifications

  • Species of Origin

    Mouse

    Expression System

    Escherichia coli

  • Affinity Tag

    His Tag (C-term)

    Storage Buffer

    Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.

  • Purity

    >98% as determined by SDS-PAGE analysis.

    Molecular weight

    The protein has a calculated MW of 14.02 kDa.
    The protein migrates about 17 kDa under reducing condition (SDS-PAGE analysis).

  • Endotoxin Level

    <0.1 EU per 1 μg of the protein by the LAL method.

    Form

    Lyophilized

• Background

  • Background

    Midkine, also known as neurite growth-promoting factor 2 (NEGF2) is a member of a small family of secreted Growth Factorss, furthermore high expression in embryo, and limb E14.5.
    Mouse midkine shares 87% sequence identity with human midkine. Midkine is a 13.5 kDa protein containing 140 amino acids, which promotes angiogenesis, cell growth, migration, and gene expression of different cell types probably via a multiprotein receptor complex consisting of several molecules.

    Synonyms

    Midkine 1 Publication, MK, Retanoic acid-responsive protein, Retinoic acid-induced differentiation factor

  • Uniprot ID

    #P12025

    Sequence Note

    Gly31-Val119

• Instruction

  • Reconstitution

    It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.

    Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

  • Stability & Storage

    This product is stable after storage at:

    • -20°C for 12 months in lyophilized state from date of receipt.
    • -20°C or -80°C for 1 month under sterile conditions after reconstitution.

    Avoid repeated freeze/thaw cycles.

• Image

  SDS-PAGE analysis of recombinant mouse Midkine.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.