Polymerase chain reaction (PCR) and reverse transcription-PCR (RT-PCR) are indispensable tools in molecular biology for amplifying specific DNA sequences. They are used in a variety of applications including gene cloning, gene expression analysis, and diagnostics. PCR utilizes short synthetic DNA primers, heat-stable DNA polymerase enzyme, and repeated cycles of replication to generate millions to billions of copies of the target DNA sequence. RT-PCR involves reverse transcription of RNA into complementary DNA (cDNA) using reverse transcriptase and amplification of the cDNA using PCR. Leadgene offers a comprehensive collection of high-quality PCR and RT-PCR reagents and master mix to ensure your results are accurate and sensitive.
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36 results
LEADSPHERE® One-Step RT-PCR Master Mix
Catalog Number：
Package：bulk
Description：Essential components for reliable RT-PCR are included in each single LEADSPHERE® One-Step RT-PCR Master Mix, which saves time for reaction set-up and reduces the risk of contamination and pipetting errors. In addition, the stabilized format facilitates shipment and storage without cooling. LEADSPHERE™ One-Step RT-PCR Master Mix includes reverse transcriptase, DNA polymerase, high-quality dNTPs, and optimized PCR buffer. This product is suitable for probe-based detection and endpoint DNA amplification. The reaction can be set up by reconstituting the LEADSPHERE™ One-Step RT-PCR Master Mix by adding PCR-grade water along with the RNA template, primers, and probe to a total volume of 20 μL.
EffiStart™ 5X Probe qPCR Master Mix (Glycerol-Free)
Catalog Number：
Package：1 mL / Customized package
Description：EffiStart™ 5X Probe qPCR Master Mix (Glycerol-Free) is an optimized reaction mix for real-time polymerase chain reaction (qPCR) kit. This product contains Hot Start Taq DNA Polymerase and is suitable for probe-based detection and formulated as a 2-fold premix. Reaction can be simply set up by adding the DNA template, primers, and probes. This master mix does not contain ROX reference dye; it offers great convenience and minimizes the risk of cross-contamination. This product is a glycerol-free formulation.
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Glycerol-free
EffiStart™ 2X One-Step Probe RT-qPCR Master Mix (Glycerol-Free)
Catalog Number：
Package：1 mL / Customized package
Description：EffiStart™ 2X One-Step Probe RT-qPCR Master Mix (Glycerol-Free) is a one-step real-time reverse transcription-polymerase chain reaction (RT-qPCR) kit developed for cDNA synthesis and real-time PCR in the same tube. This product contains Hot Start Taq DNA Polymerase and is suitable for probe-based detection and formulated as a 2-fold premix. Reaction can be simply set up by adding the RNA template, primers, and probes. This master mix does not contain ROX reference dye; it offers great convenience and minimizes the risk of cross-contamination. This product is a glycerol-free formulation.
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Glycerol-free
EffiStart™ 5X One-Step Probe RT-qPCR Master Mix (Glycerol-Free)
Catalog Number：
Package：1 mL / Customized package
Description：EffiStart™ 5X One-Step Probe RT-qPCR Master Mix (Glycerol-Free) is a one-step real-time reverse transcription-polymerase chain reaction (RT-qPCR) kit developed for cDNA synthesis and real-time PCR in the same tube. This product contains Hot Start Taq DNA Polymerase and is suitable for probe-based detection and formulated as a 5-fold premix. Reaction can be simply set up by adding the RNA template, primers, and probes. This master mix does not contain ROX reference dye; it offers great convenience and minimizes the risk of cross-contamination. This product is a glycerol-free formulation.
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Glycerol-free
EffiStart™ 2X Probe qPCR Master Mix (Glycerol-Free)
Catalog Number：
Package：1 mL / Customized package
Description：EffiStart™ 2X Probe qPCR Master Mix (Glycerol-Free) is an optimized reaction mix for real-time polymerase chain reaction (qPCR) kit. This product contains Hot Start Taq DNA Polymerase and is suitable for probe-based detection and formulated as a 2-fold premix. Reaction can be simply set up by adding the DNA template, primers, and probes. This master mix does not contain ROX reference dye; it offers great convenience and minimizes the risk of cross-contamination. This product is a glycerol-free formulation.
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Glycerol-free
M-MLV Reverse Transcriptase (Glycerol-Free)
Catalog Number：
Package：20,000 U / 50,000 U / Customized package
Description：Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase is an RNA-dependent DNA polymerase that synthesizes the first strand of complementary cDNA from a single-stranded RNA template with hybridized primer. This kit features high activity formulation of M-MLV RT and 5X reverse transcription buffer which are capable of full-length cDNA synthesis and high cDNA yields.
Expression System：Escherichia coli
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Glycerol-free
dCas9-VP64
Catalog Number：
Package：20 μg / 100 μg / Customized package
Species of Origin：Streptococcus pyogenes
Expression System：Escherichia coli
Affinity Tag：His Tag (C-term)
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Animal-free
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Carrier-free
eSpCas9 (1.1)
Catalog Number：
Package：20 μg / 100 μg / Customized package
Species of Origin：Streptococcus pyogenes
Expression System：Escherichia coli
Affinity Tag：His-MBP Tag (N-term)
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Animal-free
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Carrier-free
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Animal-free
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Carrier-free
Inorganic Pyrophosphatase (Yeast)
Catalog Number：
Package：10 U / Customized package
Description：Inorganic pyrophosphate (PPi) is generated as a reaction byproduct in many biosynthetic reactions which utilize ATP, including in vitro transcription and DNA polymerization. Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to orthophosphate (P₂O₇⁻⁴ + H₂O + PPase → 2HPO₄⁻²). PPase requires divalent metal cation (Mg²⁺) for its enzymatic activity.
Expression System：Escherichia coli
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Carrier-free
