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Leadgene Leadgene
SARS-CoV-2 Nucleocapsid Protein ELISA Kit
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Catalog Number

LDG00016E

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  • Overview

    Description

    Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) belongs to the genus Betacoronavirus, family Coronaviridae that causes coronavirus disease 2019 (COVID-19). SARS-CoV-2 is an enveloped positive-sense single-stranded RNA virus. Like other coronaviruses, SARS-CoV-2 has four structural proteins: spike, envelope, membrane and nucleocapsid. The nucleocapsid protein (NP) is the most abundant protein in coronavirus that encapsulates the genomic RNA and often used as a marker in diagnostic assays due to its high immunogenicity.
    Leadgene® SARS CoV-2 Nucleocapsid Protein ELISA kit is an enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of SARS-CoV-2 NP level in cell culture medium, nasal swab and saliva. The SARS-CoV-2 Nucleocapsid Protein ELISA kit is for research use only (RUO). Not suitable for use in therapeutic procedures.

    Test Principle

    Leadgene® SARS CoV-2 Nucleocapsid Protein ELISA kit is used to detect SARS CoV-2 NP in samples by sandwich ELISA method. This assay uses microplate pre-coated with mouse anti-SARS CoV-2 NP monoclonal antibody to the solid phase. SARS CoV-2 NP in the samples conjugates on solid phase and then react with the HRP conjugated mouse anti-SARS CoV-2 NP monoclonal antibody. Subsequent wash steps will remove unbound antibody. After incubation with substrate solution, the reaction is determined by the absorbance at 450 nm. Quantification of SARS CoV-2 NP level is accomplished by comparing the absorbance value with standard curve.

  • Specifications
    • Reactivity

      SARS-CoV-2

      Application

      Sandwich ELISA analysis

    • Detection Method

      Colorimetric

      Sensitivity

      Limit of detection (LoD): 0.171 ng/mL
      Limit of quantification (LoQ): 0.262 ng/mL

    • Specificity

      Nucleocapsid protein

      Assay Range

      0.15625-10 ng/mL

    • Sample Type

      Cell culture supernatant, Nasal swab, Saliva

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at 2-8°C for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8℃ for unopened product.

      Please refer to product manual for storage constructions.

  • Image
    Typical data The following data are for demonstration only

    Typical data
    The following data are for demonstration only

    Reagents provided and reconstitution

    Reagents provided and reconstitution

    An example of orientation of standards, blanks and samples in the stripwells microplate

    An example of orientation of standards, blanks and samples in the stripwells microplate

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  • Publication
  • Datasheet & Documents
    Datasheet
    MSDS
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Disclaimer:For Research Use or Further Manufacturing Only.

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