SARS-CoV-2 Nucleocapsid Protein, His-SUMO Tag, HEK293

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Catalog Number

LDG006PVM

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Specifications
- Species of Origin
  
  SARS-CoV-2
  
  Expression System
  
  HEK293
- Affinity Tag
  
  His-SUMO Tag (N-term)
  
  Storage Buffer
  
  Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
- Purity
  
  >98% as determined by SDS-PAGE analysis.
  
  Molecular weight
  
  The protein has a calculated MW of 57.17 kDa.
  The protein migrates as 63-75 kDa under reducing condition (SDS-PAGE analysis).
- Form
  
  Lyophilized
Background
- Background
  
  Coronaviruses (Delta B1.617.2 Variant) are enveloped positive-sense RNA viruses. Spike protein on the viral envelope and the cognate receptor on the surface of host cells that are essential for entry into host cells upon receptor binding and membrane fusion. Spike proteins are target for neutralization antibody, and mediate membrane fusion and virus entry.Trimeric spike protein is about 180 kDa, and contains two subunits, S1 and S2, mediating attachment and membrane fusion.
  
  Synonyms
  
  Nucleoprotein, N, Nucleocapsid protein, NC Protein N
- Uniprot ID
  
  #P0DTC9
  
  Sequence Note
  
  Met1-Ala419
Instruction
- Reconstitution
  
  It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
  
  Shipping
  
  The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
- Stability & Storage
  
  This product is stable after storage at:
  - -20°C for 12 months in lyophilized state from date of receipt.
  - -20°C or -80°C for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
Image

Multi-cycle kinetic analysis of Human anti-SARS-CoV & CoV-2 NP Antibody (IgG) to Human SARS-CoV-2 Nucleocapsid protein by using Biacore T200.

ELISA titration of Human anti-SARS-CoV & CoV-2 NP Antibody (A. IgG; B. IgA; C. IgM antibody).

WB analysis of recombinant Human SARS-CoV-2 nucleocapsid protein.
Lane 1: Human anti-SARS-CoV & CoV-2 NP Antibody (IgG), 0.5 μg/mL (Leadgene cat. 17901)
Lane 2: Human anti-SARS-CoV & CoV-2 NP Antibody (IgM), 0.5 μg/mL (Leadgene cat. 18301)

SDS-PAGE analysis of recombinant Human SARS-CoV-2 nucleocapsid protein.

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Publication
1. Chao CH et al. Frontiers in immunology. 2022; 13:1023943.
2. Cheng YL et al. Frontiers in immunology. 2022; 13:941923.
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Datasheet & Documents

Datasheet

MSDS

Manual

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