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Species of Origin
Swine
Expression System
Escherichia coli
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Affinity Tag
His Tag (C-term)
Storage Buffer
Lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate and 0.2 M NaCl, pH 3.5.
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Purity
>98% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 21.65 kDa.
The protein migrates as 17-25 kDa under reducing condition (SDS-PAGE analysis). -
Endotoxin Level
<0.1 EU per 1 μg of the protein by the LAL method.
Form
Lyophilized
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Specifications
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Background
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Background
Bone Morphogenetic Protein-4 (BMP-4) predicts a molecular mass of 13 kDa, is a vital regulatory molecule that functions throughout human development in mesoderm induction, tooth development, limb formation, bone induction, and fracture repair and is overexpressed in patients who have fibrodysplasia ossificans progressiva. BMP-4 is a critical signaling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP-4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog to establish a dorsal-ventral axis for the differentiation of later structures.
Uniprot ID
#NP_001094501 1
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Sequence Note
Arg227-Cys408
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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Publication
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Datasheet & Documents
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Disclaimer:For Research Use or Further Manufacturing Only.