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Species of Origin
Swine
Expression System
Escherichia coli
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Affinity Tag
His Tag (C-term)
Storage Buffer
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
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Purity
>98% as determined by SDS-PAGE analysis.
Molecular weight
The protein has a calculated MW of 21.9 kDa.
The protein migrates as 23 kDa under reducing condition (SDS-PAGE analysis). -
Activity
Measure by its ability to induce proliferation in T1165.85.2.1 cells. The ED₅₀ for this effect is <1.5 ng/mL.
Endotoxin Level
<0.1 EU per 1 μg of the protein by the LAL method.
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Form
Lyophilized
![Swine IL-6, His Tag, E. coli](https://www.leadgenebio.com/upload/website/product/model/994/images/LDG012PSEPAGE01.webp)
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Specifications
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Background
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Background
Interleukin-6 (IL-6) is a pleiotropic cytokine which plays fundamental role in the acute phase response, inflammation, bone metabolism, lymphocyte differentiation and cancer progression. Deregulation of IL-6 production was also found in several diseases, including rheumatoid arthritis, Alzheimer’s disease, autoimmune deficiency disease and different types of cancer.
Synonyms
Interleukin-6
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Uniprot ID
#P26893
Sequence Note
Arg31-Met212
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Instruction
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Reconstitution
It is recommended to reconstitute the lyophilized protein in sterile H₂O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
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Stability & Storage
This product is stable after storage at:
- -20°C for 12 months in lyophilized state from date of receipt.
- -20°C or -80°C for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
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Review
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Publication
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Datasheet & Documents
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Disclaimer:For Research Use or Further Manufacturing Only.