Language
TW
Leadgene Leadgene
T7 RNA Polymerase ELISA Kit
More Images
Catalog Number

LDG00024E

More Documents
Loading package & price...
  • Overview

    Description

    Bacteriophage T7 RNA Polymerase is a DNA-dependent RNA polymerase with high specificity for the T7 promoter. This enzyme catalyzes the 5’→3’ synthesis of RNA from DNA downstream from its promoter.
    Leadgene® T7 RNA Polymerase ELISA kit is an enzyme-linked immunosorbent assay (ELISA) for the quantitative detection of T7 RNA Polymerase level in sample solution. The T7 RNA Polymerase ELISA kit is for research use only (RUO). Not suitable for use in diagnostic or therapeutic procedures.

    Test Principle

    T7 RNA Polymerase ELISA kit is used to detect T7 RNA Polymerase in samples by sandwich ELISA method. This assay uses microplate pre-coated with mouse anti-T7 RNA Polymerase monoclonal antibody to the solid phase. T7 RNA Polymerase in the samples conjugates on solid phase and then react with the HRP conjugated mouse anti-T7 RNA Polymerase monoclonal antibody. Subsequent wash steps will residual unbound antibody. After incubation with substrate solution, the reaction is determined by the absorbance at 450 nm. Quantification of T7 RNA Polymerase protein level is accomplished by comparing the absorbance with standard curve.

  • Specifications
    • Reactivity

      Bacteriophage

      Application

      Sandwich ELISA analysis

    • Detection Method

      Colorimetric

      Sensitivity

      Limit of detection (LoD): 0.037 ng/mL
      Limit of quantification (LoQ): 0.124 ng/mL.

    • Specificity

      T7 RNA Polymerase

      Assay Range

      0.15625-10 ng/mL

    • Sample Type

      Samples contain T7 RNA Polymerase

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at 2-8°C for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8℃ for unopened product.

      Please refer to product manual for storage constructions.

  • Image
    Typical data The following data are for demonstration only

    Typical data
    The following data are for demonstration only

    Reagents provided and reconstitution

    Reagents provided and reconstitution

    An example of orientation of standards, blanks and samples in the stripwells microplate

    An example of orientation of standards, blanks and samples in the stripwells microplate

    1
    /
    3
    Total   (3)
  • Review

    Help others learn more about this product. Use the link below to share your experience.

  • Publication

    There are currently no publications. Use the link below to let us know.

  • Datasheet & Documents
    Datasheet
    MSDS
    1
    /
    1

Disclaimer:For Research Use or Further Manufacturing Only.

This site uses cookies as described in our Privacy Policy in order to improve your user experience and deliver customized advertising to you. Click “I Agree” to consent and continue.