Description
Leadgene AccuBlotTM Anti-Mouse IgG Antibody (HRP) is conjugated with high-activity HRP. In coimmunoprecipitation (co-IP) assay, denatured IgG from IP samples may show interference and result in immunoblotting at ~55 kDa (heavy chain) and ~25 kDa (light chain) by using traditional secondary antibodies. The products of AccuBlotTM series are suitable for native IgG detection.
Product Note
Recommended dilution factor:
ELISA: 1:2500-10000
WB: 1:1000-3000
Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.