AccuBlot™ Anti-Rabbit IgG (H+L) Antibody (HRP)

Catalog Number

LDG0015YE

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• Overview

  Description

  Leadgene AccuBlotTM Anti-Rabbit IgG (H+L) Antibody (HRP) is conjugated with high-activity HRP. In coimmunoprecipitation (co-IP) assay, denatured IgG from IP samples may show interference and result in immunoblotting at ~55 kDa (heavy chain) and ~25 kDa (light chain) by using traditional secondary antibodies. The products of AccuBlotTM series are suitable for native IgG detection.

  Product Note

  Recommended dilution factor:
  ELISA: 1:2500-10000
  WB: 1:1000-3000
  
  Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

• Specifications

  • Host

    Mouse

    Clonality

    Monoclonal

  • Isotype

    IgG2b

    Immunogen

    Purified rabbit IgG

  • Reactivity

    Native rabbit IgG

    Application

    ELISA, WB

  • Conjugation

    HRP

    Buffer

    Phosphate Buffered Saline containing 1% BSA, 0.03% Proclin300 and 50% glycerol, pH 7.4.

  • Specificity

    IgG

    Form

    Liquid

• Instruction

  • Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    Stability & Storage

    This product is stable after storage at:

    • 2-8°C for 2 weeks under sterile conditions from date of receipt.
    • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

    Avoid repeated freeze/thaw cycles and protect from light.
    Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

• Image

  Western blot analysis of native rabbit IgG (lane 1) and denatured rabbit IgG (lane 2), using AccuBlotᵀᴹ anti-Rabbit IgG-HRP at a 1:5000 dilution.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.