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Leadgene Leadgene
AccuBlot™ Anti-Rabbit IgG (H+L) Antibody (HRP)
Catalog Number

LDG0015YE

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  • Overview

    Description

    Leadgene AccuBlotTM Anti-Rabbit IgG (H+L) Antibody (HRP) is conjugated with high-activity HRP. In coimmunoprecipitation (co-IP) assay, denatured IgG from IP samples may show interference and result in immunoblotting at ~55 kDa (heavy chain) and ~25 kDa (light chain) by using traditional secondary antibodies. The products of AccuBlotTM series are suitable for native IgG detection.

    Product Note

    Recommended dilution factor:
    ELISA: 1:2500-10000
    WB: 1:1000-3000

    Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

  • Specifications
    • Host

      Mouse

      Clonality

      Monoclonal

    • Isotype

      IgG2b

      Immunogen

      Purified rabbit IgG

    • Reactivity

      Native rabbit IgG

      Application

      ELISA, WB

    • Conjugation

      HRP

      Buffer

      Phosphate Buffered Saline containing 1% BSA, 0.03% Proclin300 and 50% glycerol, pH 7.4.

    • Specificity

      IgG

      Form

      Liquid

  • Instruction
    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

      Stability & Storage

      This product is stable after storage at:

      • 2-8°C for 2 weeks under sterile conditions from date of receipt.
      • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

      Avoid repeated freeze/thaw cycles and protect from light.
      Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

  • Image
    Western blot analysis of native rabbit IgG (lane 1) and denatured rabbit IgG (lane 2), using AccuBlotᵀᴹ anti-Rabbit IgG-HRP at a 1:5000 dilution.

    Western blot analysis of native rabbit IgG (lane 1) and denatured rabbit IgG (lane 2), using AccuBlotᵀᴹ anti-Rabbit IgG-HRP at a 1:5000 dilution.

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  • Datasheet & Documents
    MSDS
    Manual
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