Alpha-1,3-mannosidase

Catalog Number

LDG0026RG

Datasheet

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• Overview

  Description

  Alpha-1,3-mannosidase is an enzyme that catalyzes the hydrolysis of α-1,3-mannosidic linkages in glycoproteins and glycolipids. This glycosidase is crucial in the processing and degradation of N-linked glycans, playing an essential role in glycoprotein maturation and quality control within the endoplasmic reticulum and Golgi apparatus. By trimming mannose residues, Alpha-1,3-mannosidase ensures proper folding and function of glycoproteins, impacting various biological processes including cell-cell communication, immune response, and protein stability.

• Specifications

  • Expression system

    Escherichia coli

    Concentration

    169 U/mg

  • Buffer

    20 mM Tris-HCl, 100 mM NaCl, pH 7.5

    Purity

    >85% as determined by SDS-PAGE analysis.

  • Unit Definition

    One unit is defined as the amount of α-1,3 Mannosidase required to catalyze the release of 1 nmole 4-methylumbelliferone (4MU) from 4-methylumbelliferyl α-D-mannopyranoside (4MU-Man) in 30 min at 37°C in 50 μL 1X Reaction Buffer 2 (50 mM Bis-Tris, 100mM NaCl, pH 7.0), plus 0.2 mM ZnSO4 and 1 mg/mL BSA.

    Endotoxin level

    <1 EU per 1 μg of the protein by the LAL method

• Instruction

  • Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    Stability & Storage

    This product is stable after storage at:

    • -20°C for -80°C long-term storage under sterile conditions.

    Avoid repeated free-thaw cycles.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.