Anti-DENV NS4B Antibody [Clone 24-1]

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Catalog Number

LDG0008YA

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• Overview

  Description

  Dengue virus belongs to the family Flaviviridae, genus Flavivirus and consists of four distinct serotypes (DENV1 to 4). Dengue virus is transmitted by mosquitos majorly of species Aedes aegypti and Aedes Albopictus that widespread around tropics and subtropics. NS4B is a non-enzymatic transmembrane protein, acting as an important component of the viral replication complex in the ER. The NS4B proteins can affect host immunity through protein-protein interactions.

  Product Note

  Recommended dilution factor:
  ELISA: 1:5000-20000
  WB: 1:1000-5000
  IFA: 1:500-1000
  FACS: Assay dependent
  
  Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

• Specifications

  • Host

    Mouse

    Clonality

    Monoclonal

  • Isotype

    IgG1

    Clone Name

    clone 24-1

  • Immunogen

    Dengue virus NS4B

    Reactivity

    Dengue virus

  • Application

    ELISA, WB, IFA, FACS

    Conjugation

    Unconjugated

  • Concentration

    1 mg/mL

    Buffer

    Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

  • Specificity

    NS4B

    Form

    Liquid

• Instruction

  • Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    Stability & Storage

    This product is stable after storage at:

    • 2-8°C for 2 weeks under sterile conditions from date of receipt.
    • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

    Avoid repeated freeze/thaw cycles.
    Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

• Image

  Immunofluorescence analysis of Anti-DENV NS4B Antibody [Clone 24-1]
  Control BHK cells (uninfected) and Virus (DENV 2) infection BHK cells were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with mouse anti-DENV NS4B monoclonal antibody (1:400) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488, 1:400, green) and cell nuclei were stained with Hoechst 33342 (Blue).

  Immunofluorescence analysis of Anti-DENV NS4B Antibody [Clone 24-1]
  Control Vero cells (uninfected) and Virus (DENV 2,3) infection Vero cells were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with mouse anti-DENV NS4B monoclonal antibody (1:400) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488, 1:400, green) and cell nuclei were stained with Hoechst 33342 (Blue).

  ELISA titration of Anti-DENV NS4B Antibody [Clone 24-1]
  Titration curve of anti-DENV NS4B antibody in ELISA. Red: NS4B; Black: BSA (negative control).

  Western blotting analysis of Anti-DENV NS4B Antibody [Clone 24-1]
  Western blot: Virus infection BHK cell lysates were stained with mouse anti-DENV NS4B monoclonal antibody at 1:1000 dilution.
  Lane 1: BHK cell lysate (10 μg), Lane 2: ZIKV infection (10 μg), Lane 3: DENV1 infection (10 μg), Lane 4: DENV2 infection (10 μg), Lane 5: DENV3 infection (10 μg), Lane 6: DENV4 infection (10 μg).

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.