Anti-EGFP Antibody [Clone 42-2]

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Catalog Number

LDG0008YB

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• Overview

  Description

  EGFP is a 27 kDa fluorescent protein derived from Aequorea victoria.EGFP has F64L and S65T mutations which result in brighter green fluorescence.

  Product Note

  Recommended dilution factor:
  ELISA: 1:5000-20000
  WB: 1:1000-5000
  IP: 1:500-2000
  IFA: 1:500-1000
  FACS: 1:500-1000
  
  Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

• Specifications

  • Host

    Mouse

    Clonality

    Monoclonal

  • Isotype

    IgG2a

    Clone Name

    clone 42-2

  • Immunogen

    Recombinant EGFP protein

    Application

    ELISA, WB, IP, IFA, FACS

  • Conjugation

    Unconjugated

    Concentration

    1 mg/mL

  • Buffer

    Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

    Specificity

    Recognizes EGFP

  • Form

    Liquid

• Instruction

  • Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    Stability & Storage

    This product is stable after storage at:

    • 2-8°C for 2 weeks under sterile conditions from date of receipt.
    • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

    Avoid repeated freeze/thaw cycles.
    Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

• Image

  Immunofluorescent: 293T cells were transfected with an EGFP fusion protein and fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were either natural fluorescence (green) or stained with mouse anti-EGFP monoclonal antibody (1:400) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 594, 1:400, red) and cell nuclei were stained with Hoechst 33342 (Blue).

  Western blotting analysis of anti-EGFP mAb (1:2000)
  Lane 1: 293T cell lysate
  Lane 2: EGFP cell lysate
  Lane 3: mcherry cell lysate
  Lysate at 6 μg per lane

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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