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Catalog Number

LDG0044YA

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Overview

Description

Metallothioneins (MTs) is a group of low-molecular-weight, cysteine-rich, non-enzymatic proteins, is involved in storage, transportation and binding of metals, and plays a critical role in the protection against metal toxicity and oxidative stress. Metallothionein-3 (MT-3；also known as Growth Inhibitory Factor, GIF), a member of the MTs family, is mainly expressed in the central nervous system, can protect neuronal cells from oxidative stress via its anti-oxidant properties. MT-3 is abnormally under-expressed in the brains of Alzheimer's disease patients.

Product Note

Recommended dilution factor:
ELISA: 1:5000-20000
WB: 1:1000-10000
IFA: 1:100-1000
FACS: Assay dependent

Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.
Specifications
- Host
  
  Mouse
  
  Clonality
  
  Monoclonal
- Isotype
  
  IgG1
  
  Clone Name
  
  clone 50-1
- Immunogen
  
  GIF
  
  Reactivity
  
  Human
- Application
  
  ELISA, WB, IFA, FACS
  
  Conjugation
  
  Unconjugated
- Concentration
  
  1 mg/mL
  
  Buffer
  
  Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.
- Specificity
  
  GIF
  
  Form
  
  Liquid
Instruction
- Shipping
  
  The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.
  Stability & Storage
  
  This product is stable after storage at:
  - 2-8°C for 2 weeks under sterile conditions from date of receipt.
  - -20°C or -80°C for 12 months under sterile conditions from date of receipt.
  Avoid repeated freeze/thaw cycles.
  Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.
Image

Immunofluorescence analysis of Anti-GIF Antibody [Clone 50-1]
HeLa cells were fixed in 4% PFA, permeabilized with PBS containing 0.1%
Triton X-100. Cells were stained with mouse anti-GIF monoclonal antibody
(1:100) followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488,
1:200, green) and cell nuclei were stained with Hoechst 33342 (Blue).

FACS analysis of Anti-GIF Antibody [Clone 50-1]
K562 cells were stained with mouse anti-GIF monoclonal antibody at 2 μg/ml
(red) and without antibody control (black).

ELISA titration of Anti-GIF Antibody [Clone 50-1]
Titration curve of anti-GIF antibody in ELISA. Red: GIF; Black: BSA (negative
control).

Western blotting analysis of Anti-GIF Antibody [Clone 50-1]
Recombinant proteins and mouse stomach lysates were stained with mouse
anti-GIF monoclonal antibody at 1:5000 dilution. Lane 1: Recombinant GIF
protein (100 ng). Lane 2: Mouse stomach lysate (30 μg). Lane 3: Human
gastric juice (10 μL).

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Datasheet & Documents

Datasheet

MSDS

Manual

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