Anti-HDGF Antibody [Clone 83-5]

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Catalog Number

LDG0040YA

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• Overview

  Description

  Hepatoma-derived growth factor (HDGF；also known as high mobility group protein 1-like 2, HMG-1L2) is a heparin-binding protein that is ubiquitously expressed in normal tissues and abnormally expressed in various malignancies. HDGF has various intracellular functions and localizes to the nucleus, and thus it is characterized as a mitogen for many cell types. As its promoting growth, suppressing differentiation and exhibiting angiogenic properties, HDGF plays a critical role in tumor progression.

  Product Note

  Recommended dilution factor:
  ELISA: 1:5000-20000
  WB: 1:1000-10000
  IFA: 1:200-1000
  FACS: Assay dependent
  
  Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

• Specifications

  • Host

    Mouse

    Clonality

    Monoclonal

  • Isotype

    IgG1

    Clone Name

    clone 83-5

  • Immunogen

    HDGF

    Reactivity

    Human

  • Application

    ELISA, WB, IFA, FACS

    Conjugation

    Unconjugated

  • Concentration

    1 mg/mL

    Buffer

    Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

  • Specificity

    HDGF

    Form

    Liquid

• Instruction

  • Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    Stability & Storage

    This product is stable after storage at:

    • 2-8°C for 2 weeks under sterile conditions from date of receipt.
    • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

    Avoid repeated freeze/thaw cycles.
    Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

• Image

  Immunofluorescence analysis of Anti-HDGF Antibody [Clone 83-5]
  Cells were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton
  X-100. Cells were stained with anti-HDGF monoclonal antibody (1:200)
  followed by secondary antibodies (goat anti-Mouse IgG-iFluor 488, 1:200,
  green) and cell nuclei were stained with Hoechst 33342 (Blue).

  Western blotting analysis of Anti-HDGF Antibody [Clone 83-5]
  Tissue lysate was stained with anti-HDGF monoclonal antibody at 1:5000
  dilution. Lane 1: Mouse liver lysate (50 μg). Lane 2: Rat liver lysate (50 μg).

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.