Anti-His Tag Antibody [Clone 14-4]

Catalog Number

LDG0001YB

Datasheet

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• Overview

  Description

  Mouse anti-His tag mAb is specific to 6X His tag, which can be applied to several immunoassays, including ELISA, WB, IP, IFA, and FACS. This antibody has been identified in several sources (bacteria, yeast, and mammalian cells) of His tag fusion protein.

  Product Note

  Recommended dilution factor:
  ELISA: 1:5000-20000
  WB: 1:1000-5000
  IP: 1:500-2000
  IFA: 1:500-1000
  FACS: 1:500-1000
  
  Note: Working dilution for specific application should be determined by the investigator to obtain the best conditions.

• Specifications

  • Host

    Mouse

    Clonality

    Monoclonal

  • Isotype

    IgG1

    Clone Name

    clone 14-4

  • Immunogen

    6X His tag fusion protein

    Application

    ELISA, WB, IP, IFA, FACS

  • Conjugation

    Unconjugated

    Concentration

    1 mg/mL

  • Buffer

    Phosphate Buffered Saline containing 0.03% ProClin 300, pH 7.4.

    Specificity

    Recognizes 6X His tag on either the N- or C-terminus of recombinant fusion protein.

  • Form

    Liquid

• Instruction

  • Shipping

    The product is shipped with polar packs. Upon receipt, store it immediately at -20°C or lower for long term storage.

    Stability & Storage

    This product is stable after storage at:

    • 2-8°C for 2 weeks under sterile conditions from date of receipt.
    • -20°C or -80°C for 12 months under sterile conditions from date of receipt.

    Avoid repeated freeze/thaw cycles.
    Suggestion: Divide antibody into several vials. Keep only vials for usage at 2-8°C.

• Image

  Western blotting analysis of anti-His mAb (1:5000)
  Lane 1: 293T whole cell lysate-untransfected
  Lane 2: 293T whole cell lysate-transfected
  Lysate/proteins at 20 ug per lane

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• Review

  

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• Publication

  1. Cheng YL et al. Frontiers in immunology. 2022; 13:941923.
  2. Yang YH et al. Viruses. 2022; 14(5):925.
  3. Lai YC et al. Frontiers in immunology. 2022; 13:868724.

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• Datasheet & Documents

  Datasheet

  MSDS

  Manual

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Disclaimer：For Research Use or Further Manufacturing Only.